Diodontidae: M I A3

Diodon holocanthus Linnaeus. 1758

Balloon porcupinefish   

Egg diameter in µm	Number of oil globules	Diameter of oil globule in µm	Yolk texture	Perivitelline space	Position of oil globule at hatch	Gut length   at eye- pigment stage	Myomeres
1610-1870	multiple	NA	clear	narrow	stern	70% of NL	20-21

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Egg: The early egg of this species has a distinct red sheen to the chorion (A). The late embryo and yolksac are covered in white pigment spots (C). Incubation is 70-90 hours. One specimen was seen with a single, amber oil globule of 215µm..

Larva: The early larva is covered in fine white spots (D), which persist to 5 days, when the larva turns blue-grey (E), and to 7 days, when the larva is dark above and yellow below (F & G).  D: NH, E: 5 days, F: 6 days, G: 7 days (24°C).

Shao et al (2001) show Diodon holocanthus as having an egg of this size. Six larvae have been sequenced, and they match a D. holocanthus adult sequence in the BOLD Archive. No locally collected adult fish have been barcoded.

Linked samples	Offshore	Inshore
Eggs	19	26
Hits	14	10

Like MIA1A, this is a summer spawner in the Park Rynie area (blue graph). The Park Rynie linked samples had more eggs inshore (58%), but numbers are low. See Section 7.3 and Table 1 of the Introductory Notes, for more information on the linked samples.